A single gene in Fusarium oxysporum limits host range.
Identifieur interne : 000224 ( Main/Exploration ); précédent : 000223; suivant : 000225A single gene in Fusarium oxysporum limits host range.
Auteurs : Jiming Li [Pays-Bas] ; Like Fokkens [Pays-Bas] ; Martijn Rep [Pays-Bas]Source :
- Molecular plant pathology [ 1364-3703 ] ; 2020.
Abstract
Fusarium oxysoporum f. sp. radicis-cucumerinum (Forc) is able to cause disease in cucumber, melon, and watermelon, while F. oxysporum f. sp. melonis (Fom) can only infect melon plants. Earlier research showed that mobile chromosomes in Forc and Fom determine the difference in host range between Forc and Fom. By closely comparing these pathogenicity chromosomes combined with RNA-sequencing data, we selected 11 candidate genes that we tested for involvement in the difference in host range between Forc and Fom. One of these candidates is a putative effector gene on the Fom pathogenicity chromosome that has nonidentical homologs on the Forc pathogenicity chromosome. Four independent Forc transformants with this gene from Fom showed strongly reduced or no pathogenicity towards cucumber, while retaining pathogenicity towards melon and watermelon. This suggests that the protein encoded by this gene is recognized by an immune receptor in cucumber plants. This is the first time that a single gene has been demonstrated to determine a difference in host specificity between formae speciales of F. oxysporum.
DOI: 10.1111/mpp.13011
PubMed: 33146465
Affiliations:
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Earlier research showed that mobile chromosomes in Forc and Fom determine the difference in host range between Forc and Fom. By closely comparing these pathogenicity chromosomes combined with RNA-sequencing data, we selected 11 candidate genes that we tested for involvement in the difference in host range between Forc and Fom. One of these candidates is a putative effector gene on the Fom pathogenicity chromosome that has nonidentical homologs on the Forc pathogenicity chromosome. Four independent Forc transformants with this gene from Fom showed strongly reduced or no pathogenicity towards cucumber, while retaining pathogenicity towards melon and watermelon. This suggests that the protein encoded by this gene is recognized by an immune receptor in cucumber plants. This is the first time that a single gene has been demonstrated to determine a difference in host specificity between formae speciales of F. oxysporum.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33146465</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1364-3703</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Nov</Month><Day>04</Day></PubDate></JournalIssue><Title>Molecular plant pathology</Title><ISOAbbreviation>Mol Plant Pathol</ISOAbbreviation></Journal><ArticleTitle>A single gene in Fusarium oxysporum limits host range.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1111/mpp.13011</ELocationID><Abstract><AbstractText>Fusarium oxysoporum f. sp. radicis-cucumerinum (Forc) is able to cause disease in cucumber, melon, and watermelon, while F. oxysporum f. sp. melonis (Fom) can only infect melon plants. Earlier research showed that mobile chromosomes in Forc and Fom determine the difference in host range between Forc and Fom. By closely comparing these pathogenicity chromosomes combined with RNA-sequencing data, we selected 11 candidate genes that we tested for involvement in the difference in host range between Forc and Fom. One of these candidates is a putative effector gene on the Fom pathogenicity chromosome that has nonidentical homologs on the Forc pathogenicity chromosome. Four independent Forc transformants with this gene from Fom showed strongly reduced or no pathogenicity towards cucumber, while retaining pathogenicity towards melon and watermelon. This suggests that the protein encoded by this gene is recognized by an immune receptor in cucumber plants. This is the first time that a single gene has been demonstrated to determine a difference in host specificity between formae speciales of F. oxysporum.</AbstractText><CopyrightInformation>© 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jiming</ForeName><Initials>J</Initials><Identifier Source="ORCID">https://orcid.org/0000-0001-7443-7748</Identifier><AffiliationInfo><Affiliation>Molecular Plant Pathology, University of Amsterdam, Amsterdam, Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fokkens</LastName><ForeName>Like</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Molecular Plant Pathology, University of Amsterdam, Amsterdam, Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rep</LastName><ForeName>Martijn</ForeName><Initials>M</Initials><Identifier Source="ORCID">https://orcid.org/0000-0003-3608-6283</Identifier><AffiliationInfo><Affiliation>Molecular Plant Pathology, University of Amsterdam, Amsterdam, Netherlands.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>016.veni.181.090</GrantID><Agency>Nederlandse Organisatie voor Wetenschappelijk Onderzoek</Agency><Country></Country></Grant><Grant><GrantID>201504910768</GrantID><Agency>Chinese Scholarship Council</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Plant Pathol</MedlineTA><NlmUniqueID>100954969</NlmUniqueID><ISSNLinking>1364-3703</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Fusarium oxysoporum f. sp. radicis-cucumerinum</Keyword><Keyword MajorTopicYN="N">Fusarium oxysporum f. sp. melonis</Keyword><Keyword MajorTopicYN="N">cucurbits</Keyword><Keyword MajorTopicYN="N">effector-triggered immunity (ETI)</Keyword><Keyword MajorTopicYN="N">host range</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>03</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>07</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>4</Day><Hour>8</Hour><Minute>45</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33146465</ArticleId><ArticleId IdType="doi">10.1111/mpp.13011</ArticleId></ArticleIdList><ReferenceList><Title>REFERENCES</Title><Reference><Citation>Biju, V.C., Fokkens, L., Houterman, P.M., Rep, M. and Cornelissen, B.J.C. 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Molecular Plant Pathology, 17, 1455-1466.</Citation></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country><region><li>Hollande-Septentrionale</li></region><settlement><li>Amsterdam</li></settlement><orgName><li>Université d'Amsterdam</li></orgName></list><tree><country name="Pays-Bas"><region name="Hollande-Septentrionale"><name sortKey="Li, Jiming" sort="Li, Jiming" uniqKey="Li J" first="Jiming" last="Li">Jiming Li</name></region><name sortKey="Fokkens, Like" sort="Fokkens, Like" uniqKey="Fokkens L" first="Like" last="Fokkens">Like Fokkens</name><name sortKey="Rep, Martijn" sort="Rep, Martijn" uniqKey="Rep M" first="Martijn" last="Rep">Martijn Rep</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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